Abstract: Sperm cryopreservation is a powerful tool addressing global challenges like human infertility, animal breeding, biodiversity loss, and biomedical research. While liquid nitrogen (LN2) at -196°C has long been the standard for sperm storage, it poses cost, safety, and logistical concerns. With rising infertility rates and growing use of assisted reproductive technologies like IVF and ICSI, frozen-thawed sperm is widely used in both human and animal applications. Additionally, as biodiversity faces threats from climate change and pollution, sperm banking has become essential for conserving genetic material across species.
To overcome LN2’s limitations, researchers now propose using ultra-low freezers at -80°C as a practical alternative. These are safer, more affordable, and accessible, especially for low- and middle-income regions. Studies show that sperm stored at -80°C retain fertilization potential, and the use of cryoprotectants and antioxidants further protects sperm quality. This method also supports sustainable, decentralized biobanking, including for space-based research where LN2 isn’t viable. This shift could transform the future of reproductive science by making sperm preservation more inclusive, efficient, and eco-friendly.
Full article available here: From -196°C to -80°C: redefining standards in sperm biobanking technology – ScienceDirect
